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KMID : 0377619740270040345
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Korean Jungang Medical Journal
1974 Volume.27 No. 4 p.345 ~ p.350
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Morphological Response of Enterochromaffin Cells of Mice to Systemic Anesthetics
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Abstract
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This experiment was performed to study morphological responses of enterochromaffin cells in the duodenum and colon of mice under the general anesthesia.
Eighty healthy adult mice weighing about 20g each were divided into two groups; normal and anesthetized. General anesthetics used in this experiment were diethyl ether, chloroform, and halothane as inhalation anesthetics, and thiopental and propanidid as injection anesthetics. Inhalation anesthesia was done by open drop method and injection anesthesia was maintained in surgical anesthesia by a single intraperitoneal injection of 2. 5mg in cases of thiopental and by a initial intraperitoneal injection of 10mg and maintenance dose of 5mg every 15 minutes in cases of propanidid.
Anesthetized animals were sacrificed on the 30th minutes, 1st and 2nd hour after the induction. Mucosal specimens from the duodenum and the colon of all the experimental mice were fixed in 10% neutral formalin solution, embedded. In paraffin wax, sectioned at a thickness of 6 p, and impregnated by Masson-Hamperl´s silvering method. The numerical changes of enterochromaffin cells were compared with the numbers of the cells counted in mucosae of 3.0mm width and 6 ,a thickness, and the changes of granularity of the cells were evaluated semiquantitatively by the granulation index, arithmetically weighted to 3-cell types classified according to the degree of granularity of the cells.
The results were as follows:
1) In the normal duodenal mucosae, granulation cell index and enterochromaffin granulation index of enterochromaff in cells were 40. 9±7. 90, 79.8±16.09, respectively and in the colonic mucosae, 33. 4±4. 24, 67. 6±3. 86, respectively.
2) Under general anesthesia, there were little changes in granulation cell index and enterochromaffin granulation index and also noticeable changes in shape and distribution of the cell and in distribution of their specific granules were not observed.
3) Judging from the above findings, the enterochromaffin ceIIs were little affection under the general anesthesia.
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